Genotypic Variability in Soybean [Glycine max (L.) Merrill] through Agrobacterium-Mediated Transformation
نویسندگان
چکیده
منابع مشابه
Improvement of qualitative and quantitative traits in soybean [Glycine Max (L.) Merrill] through gamma irradiation
Gamma irradiation was used at different doses (80, 160 and 240 Gy) on Glycine Max (L.) Merrill cv. Hill homogenous seeds. A single suitable M2 plant was selected and evaluated at M3 and M4 along with its parent and three other varieties as control in RCBD experiment in Sari Agricultural Sciences and Natural Resources University’s experimental field. M-8...
متن کاملSoybean [Glycine max (L.) Merr].
In this chapter we describe an Agrobacterium tumefaciens transformation method of soybean that utilizes mature half seeds and regeneration from the cotyledonary node region. This method results in fertile transformed soybean plants and transgenic seed in approximately 9 months. Using mature half seeds as starting material has proven to be a reliable method that does not require additional wound...
متن کاملGenetic variation in an inbred plant: variation in tissue cultures of soybean [Glycine max (L.) Merrill].
Although soybean [Glycine max (L.) Merrill] grows as an inbreeding, generally homozygous, plant, the germplasm of the species contains large amounts of genetic variation. Analysis of soybean DNA has indicated that variation of RFLP (restriction fragment length polymorphism) markers within the species usually entails only two alleles at any one locus and that mixtures of such dimorphic loci acco...
متن کاملimprovement of qualitative and quantitative traits in soybean [glycine max (l.) merrill] through gamma irradiation
gamma irradiation was used at different doses (80, 160 and 240 gy) on glycine max (l.) merrill cv. hill homogenous seeds. a single suitable m2 plant was selected and evaluated at m3 and m4 along with its parent and three other varieties as control in rcbd experiment in sari agricultural sciences and natural resources university’s experimental field. m-80-709 and m-160-3429 mutant lines were sig...
متن کاملA Rapid Method to Analyze Saponin Precursors in Soybean (Glycine max (L.) Merrill)
A rapid and efficient analytical method was developed to distinguish and to quantify two major saponin precursors in soybeans; soyasapogenol A, contributing to the undesirable sensory characteristics, and soyasapogenol B, representing group B and E saponins, which have chemoprotective properties. Aqueous ethanolic extraction (80 % v/v) yielded the highest recovery of saponin from finely ground ...
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ژورنال
عنوان ژورنال: Plant Tissue Culture and Biotechnology
سال: 2020
ISSN: 1818-8745,1817-3721
DOI: 10.3329/ptcb.v30i2.50693